rabbit polyclonal antibodies against cd68  (Millipore)

Journal: BMC Genomics

Article Title: Differential gene expression and immune cell infiltration in maedi-visna virus-infected lung tissues

doi: 10.1186/s12864-024-10448-2

Figure Lengend Snippet: Immunostaining of CD68-positive macrophages and CD19‐positive B cells infiltrating the lung. ( A ) Control samples for CD68 immunostaining. ( B ) CD68 immunostaining of lungs from Maedi-visna virus (MVV)-infected sheep. ( C ) Control samples for CD19 immunostaining. ( D ) CD19 immunostaining of lungs from MVV-infected sheep (red bar, 50 μm; green bar, 40 μm)

Article Snippet: The tissues were incubated with a 1:200 dilution of CD4 polyclonal antibody (19068-1-AP, Proteintech, Wuhan, China), rabbit polyclonal CD8 alpha (ab4055, Abcam, Cambridge, USA), CD19 rabbit mAb (A19013, ABclonal Technology Co., Ltd.), and rabbit polyclonal antibody against CD68 (DF7518, Affinity Biosciences) overnight at 4 °C.

Techniques: Immunostaining, Control, Virus, Infection

Journal: Journal of Translational Medicine

Article Title: Parathyroid hormone stimulates bone regeneration in an atrophic non-union model in aged mice

doi: 10.1186/s12967-023-04661-y

Figure Lengend Snippet: A Representative immunohistochemical images of CD31-positive microvessels (arrowheads) within the callus tissue of controls and PTH-treated mice at 2 and 10 weeks after surgery. Scale bars: 25 μm. B CD31-positive microvessels/HPF within the callus tissue of controls (white bars, n = 5 at 2 weeks after surgery, n = 9 at 10 weeks after surgery) and PTH-treated mice (black bars, n = 5 at 2 weeks after surgery, n = 9 at 10 weeks after surgery) at 2 and 10 weeks after surgery, as assessed by immunohistochemical analysis. Mean ± SEM; *p < 0.05 vs. control. C Representative immunohistochemical images of MPO-positive cells (arrowheads) within the callus tissue of controls and PTH-treated mice at 2 and 10 weeks after surgery. Scale bars: 25 μm. D MPO-positive cells/HPF within the callus tissue of controls (white bars, n = 5 at 2 weeks after surgery, n = 9 at 10 weeks after surgery) and PTH-treated mice (black bars, n = 5 at 2 weeks after surgery, n = 9 at 10 weeks after surgery) at 2 and 10 weeks after surgery, as assessed by immunohistochemical analysis. Mean ± SEM; *p < 0.05 vs. control. E Representative immunohistochemical images of CD68-positive cells (arrowheads) within the callus tissue of controls and PTH-treated mice at 2 and 10 weeks after surgery. Scale bars: 25 μm. F CD68-positive cells/HPF within the callus tissue of controls (white bars, n = 5 at 2 weeks after surgery, n = 9 at 10 weeks after surgery) and PTH-treated mice (black bars, n = 5 at 2 weeks after surgery, n = 9 at 10 weeks after surgery) at 2 and 10 weeks after surgery, as assessed by immunohistochemical analysis. Mean ± SEM; *p < 0.05 vs. control

Article Snippet: To detect the neutrophilic granulocyte marker myeloperoxidase (MPO) and the macrophage marker CD68, sections were stained with a polyclonal rabbit anti-mouse antibody against MPO (1:100; Abcam) and a polyclonal rabbit anti-mouse antibody against CD68 (1:100; Abcam).

Techniques: Immunohistochemistry

Journal: Scientific Reports

Article Title: Proliferative diabetic retinopathy transcriptomes reveal angiogenesis, anti-angiogenic therapy escape mechanisms, fibrosis and lymphatic involvement

doi: 10.1038/s41598-021-97970-5

Figure Lengend Snippet: Inflammation and wound healing are involved in PDR and RRD-PVR. ( a ) Bar chart showing GO-terms related to inflammation and wound healing, based on the DEGs upregulated in PDR. Numbers beside bars indicate number of DEGs in GO-term versus the total number of genes in the GO-term. ( b ) Heatmap of log 2 -transformed normalized read counts of DEGs in GO-term Platelet activation . ( c ) Heatmap of log 2 -transformed normalized read counts of DEGs in IPA canonical pathway Leukocyte extravasation signalling. ( d ) Representative light micrographs of immunohistochemistry for CD45 in excised PDR and RRD-PVR tissue. ( e–f ) Heatmap of log 2 -transformed normalized read counts of DEGs in T-cell and B-cell signatures ( e ), and in macrophage, monocyte, and neutrophil cell signatures ( f ). ( g ) Representative light micrographs of immunohistochemistry of CD68 in PDR and RRD-PVR tissue. Scale bars, 10 µm (left), 5 µm (magnified insets).

Article Snippet: Mouse monoclonal antibodies against CD31 (1:100; M0823; Dako), Notch-1 (1:50; sc-376403; Santa Cruz; CA, USA), SOX18 (1:100; sc-166025; Santa Cruz,) and CD45 (1:100; M070129-2; Dako) and rabbit polyclonal antibodies against CD68 (HPA_048982; 1:3000; Sigma) were used.

Techniques: Transformation Assay, Activation Assay, Immunohistochemistry

Journal: Antioxidants

Article Title: Oxidative Stress and Inflammatory Markers in Abdominal Aortic Aneurysm

doi: 10.3390/antiox10040602

Figure Lengend Snippet: Immune infiltrate in AAA. ( A – D ) Representative images of immunostaining assays performed in abdominal aorta sections from AAA patients and donors targeting CD3 (T-lymphocyte s), CD68 (macrophages), CD19 (B-lymphocytes) and elastase (Neutrophils), respectively ( n = 10; Scale bars: 50 µm). AAA (-) indicates negative control for immunohistochemistry. Arrows indicate the positively stained cells.

Article Snippet: Double-fluorescence immunostaining was performed by sequentially incubating the sections with mouse polyclonal antibodies against CD206 (ab64693) or CD80 (ab254579) at 4 °C overnight followed by the incubation with a rabbit polyclonal antibody against CD68 (M0876, Dako).

Techniques: Immunostaining, Negative Control, Immunohistochemistry, Staining